Hotspot

ndhA

SSC · 123036-125208 · 2173 bp

Back to Trachycarpus

Pi 0.00175

MarkerSeek score 41.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2173
variable_sites: 10
pi: 0.00175
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTCACCATAGAGAACA	TGGGAGAAGTTGATCGTTGA	47.6	45.0	58.1	56.5	2285-2304 bp	1.0	60.4
ndhA_p2	TGAACCCAGTCACCATAGAGA	TGGGAGAAGTTGATCGTTGA	47.6	45.0	57.8	56.5	2288-2307 bp	1.0	59.7
ndhA_p3	ATTCATGAACCCAGTCACCA	TGGGAGAAGTTGATCGTTGA	45.0	45.0	56.7	56.5	2293-2312 bp	1.0	59.0
ndhA_p4	TTCATGAACCCAGTCACCAT	TGGGAGAAGTTGATCGTTGA	45.0	45.0	56.7	56.5	2292-2311 bp	1.0	59.0
ndhA_p5	ACCCAGTCACCATAGAGAACA	TGGGAGAAGTTGATCGTTGAA	47.6	42.9	58.1	57.2	2285-2304 bp	1.0	58.4