Hotspot

psaC-ndhE

SSC · 120269-121032 · 764 bp

Back to Trachycarpus

Pi 0.00929

MarkerSeek score 63.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 764
variable_sites: 14
pi: 0.00929
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	CTTTGTCATAGCTGTTGCGG	50.0	50.0	58.1	57.5	739-1003 bp	1.0	79.9
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.1	57.4	716-980 bp	1.0	79.2
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.1	57.4	715-979 bp	1.0	79.2
psaC-ndhE_p4	CAATACATCTGTGGGGCAGG	CTTTGTCATAGCTGTTGCGG	55.0	50.0	58.3	57.5	715-979 bp	1.0	78.4
psaC-ndhE_p5	CCAATACATCTGTGGGGCAG	CTTTGTCATAGCTGTTGCGG	55.0	50.0	58.3	57.5	716-980 bp	1.0	78.4