Hotspot

accD-psaI

LSC · 60885-61769 · 885 bp

Back to Trachycarpus

Pi 0.00806

MarkerSeek score 59.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 18
pi: 0.00806
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	984-1001 bp	1.0	83.4
accD-psaI_p2	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	985-1002 bp	1.0	83.4
accD-psaI_p3	CCACGGTTTCTTTCCCTTGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	990-1007 bp	1.0	82.8
accD-psaI_p4	TCCACGGTTTCTTTCCCTTG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	991-1008 bp	1.0	82.8
accD-psaI_p5	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	990-1007 bp	1.0	82.7