Hotspot

rbcL-accD

LSC · 58623-59408 · 786 bp

Back to Trachycarpus

Pi 0.00127

MarkerSeek score 49.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 3
pi: 0.00127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCAAATGGAGCCCTGAACTA	GGTTCTTCACTTCCACTGCT	50.0	50.0	57.9	57.7	972-1003 bp	1.0	80.1
rbcL-accD_p2	TAGCAAATGGAGCCCTGAAC	GGTTCTTCACTTCCACTGCT	50.0	50.0	57.9	57.7	974-1005 bp	1.0	80.1
rbcL-accD_p3	TGAAGCTAGCAAATGGAGCC	GGTTCTTCACTTCCACTGCT	50.0	50.0	58.2	57.7	980-1011 bp	1.0	79.1
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	GGTTCTTCACTTCCACTGCT	50.0	50.0	58.3	57.7	983-1014 bp	1.0	79.0
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	GGTTCTTCACTTCCACTGCT	50.0	50.0	58.3	57.7	984-1015 bp	1.0	79.0