Hotspot

ndhC-trnM-CAU

LSC · 51516-54243 · 2728 bp

Back to Trachycarpus

Pi 0.000758
MarkerSeek score 46.40
Primer available yes
Label ndhC-trnM-CAU

Feature Metadata

label_name
ndhC-trnM-CAU
feature_type
igs
region
LSC
length_bp
2728
variable_sites
5
pi
0.000758
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAU_p1 TCCTTCACTAACCGGTGCTA CCCGCCGTATGAAAGCAATA 50.0 50.0 58.1 58.4 2851-2883 bp 1.0 77.7
ndhC-trnM-CAU_p2 TAACCGGTGCTAAAACTCCG CCCGCCGTATGAAAGCAATA 50.0 50.0 57.9 58.4 2843-2875 bp 1.0 77.6
ndhC-trnM-CAU_p3 TCCTTCACTAACCGGTGCTA ATCACTCAATTGGCTTCGGT 50.0 45.0 58.1 57.5 2913-2945 bp 1.0 76.9
ndhC-trnM-CAU_p4 TAACCGGTGCTAAAACTCCG ATCACTCAATTGGCTTCGGT 50.0 45.0 57.9 57.5 2905-2937 bp 1.0 76.9
ndhC-trnM-CAU_p5 TCCTTCACTAACCGGTGCTA CCGCCGTATGAAAGCAATAC 50.0 50.0 58.1 57.3 2850-2882 bp 1.0 75.6