Hotspot

psbK-psbI

LSC · 7894-8292 · 399 bp

Back to Trachycarpus

Pi 0.00451

MarkerSeek score 31.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 4
pi: 0.00451
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	AGGATTACGCCCTGGATCAT	50.0	50.0	57.9	58.3	525 bp	1.0	78.0
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	522 bp	1.0	76.0
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	523 bp	1.0	76.0
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	524 bp	1.0	76.0
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	GGATTACGCCCTGGATCATT	50.0	50.0	57.9	57.4	524 bp	1.0	75.6