Hotspot

rps16

LSC · 4968-6067 · 1100 bp

Back to Trachelospermum

Pi 0.00273

MarkerSeek score 50.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1100
variable_sites: 3
pi: 0.00273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGTTATATACAAATCACTACCCCC	ACGATGTCTACGAGCCAATG	37.5	50.0	55.5	57.8	1217-1225 bp	1.0	40.2
rps16_p2	AGTTATATACAAATCACTACCCCC	CGATGTCTACGAGCCAATGA	37.5	50.0	55.5	57.5	1216-1224 bp	1.0	40.2
rps16_p3	AAGTTATATACAAATCACTACCCCC	ACGATGTCTACGAGCCAATG	36.0	50.0	56.2	57.8	1218-1226 bp	1.0	40.2
rps16_p4	GTTATATACAAATCACTACCCCC	ACGATGTCTACGAGCCAATG	39.1	50.0	54.0	57.8	1216-1224 bp	1.0	40.2
rps16_p5	AAGTTATATACAAATCACTACCCCC	CGATGTCTACGAGCCAATGA	36.0	50.0	56.2	57.5	1217-1225 bp	1.0	40.2