Hotspot

psbC

LSC · 35065-36486 · 1422 bp

Back to Trachelospermum

Pi 0.00281

MarkerSeek score 51.70

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 4
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TCTCAGGAAATTCGAGCAGC	AGAATGACCCGATCCCAAGA	50.0	50.0	58.0	58.1	1609 bp	1.0	79.1
psbC_p2	AAATTCGAGCAGCGGAAGAT	AGAATGACCCGATCCCAAGA	45.0	50.0	58.0	58.1	1602 bp	1.0	79.0
psbC_p3	TCTCAGGAAATTCGAGCAGC	TGACCCGATCCCAAGATGTA	50.0	50.0	58.0	57.8	1605 bp	1.0	78.7
psbC_p4	AAATTCGAGCAGCGGAAGAT	TGACCCGATCCCAAGATGTA	45.0	50.0	58.0	57.8	1598 bp	1.0	78.6
psbC_p5	GCAGCGGAAGATCCAGAATT	AGAATGACCCGATCCCAAGA	50.0	50.0	58.3	58.1	1594 bp	1.0	76.6