Hotspot

psbA

LSC · 376-1437 · 1062 bp

Back to Trachelospermum

Pi 0.00282

MarkerSeek score 49.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCCTATACACCAAGACCGGG	AACAAGTCCGCCATTCTCTC	55.0	50.0	58.2	57.9	1171 bp	1.0	77.7
psbA_p2	TCCTATACACCAAGACCGGG	TGGGAGTCCCTGACGATTAA	55.0	50.0	58.2	57.8	1124 bp	1.0	76.6
psbA_p3	TCCTATACACCAAGACCGGG	TTGGGAGTCCCTGACGATTA	55.0	50.0	58.2	57.8	1125 bp	1.0	76.6
psbA_p4	TCCTATACACCAAGACCGGG	TAGAGAATGTGTGCGCTTGG	55.0	50.0	58.2	58.3	1141 bp	1.0	76.3
psbA_p5	TCCTATACACCAAGACCGGG	ACAAGTCCGCCATTCTCTCT	55.0	50.0	58.2	58.7	1170 bp	1.0	72.6