Hotspot

cemA

LSC · 62997-63677 · 681 bp

Back to Trachelospermum

Pi 0.00587

MarkerSeek score 62.10

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 681
variable_sites: 4
pi: 0.00587
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	CGCATAGAGTCGACGAATGA	AGTAGCAAACCTTCGAATTGGA	50.0	40.9	57.6	58.0	788 bp	1.0	61.2
cemA_p2	TCGCATAGAGTCGACGAATG	AGTAGCAAACCTTCGAATTGGA	50.0	40.9	57.6	58.0	789 bp	1.0	61.2
cemA_p3	GACGAATGAGGTGGGTTGAT	AGTAGCAAACCTTCGAATTGGA	50.0	40.9	57.6	58.0	777 bp	1.0	61.1
cemA_p4	CGCATAGAGTCGACGAATGA	AGCAAACCTTCGAATTGGAA	50.0	40.0	57.6	55.9	785 bp	1.0	60.1
cemA_p5	TCGCATAGAGTCGACGAATG	AGCAAACCTTCGAATTGGAA	50.0	40.0	57.6	55.9	786 bp	1.0	60.1