Hotspot

ccsA

SSC · 115786-116754 · 969 bp

Back to Trachelospermum

Pi 0.00722

MarkerSeek score 50.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 7
pi: 0.00722
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGGCTTCTTCAATCTCGACG	GTACCCGGATTTCGCTCTTT	50.0	50.0	58.0	58.0	1512-1521 bp	1.0	80.3
ccsA_p2	TGGCTTCTTCAATCTCGACG	TTGCATTACCGGGTATGAGC	50.0	50.0	58.0	58.0	1786-1795 bp	1.0	80.1
ccsA_p3	TGGCTTCTTCAATCTCGACG	TATCTTGACGAAATGGGCGG	50.0	50.0	58.0	58.1	1865-1874 bp	1.0	80.0
ccsA_p4	TGGCTTCTTCAATCTCGACG	TGACGAAATGGGCGGAATAG	50.0	50.0	58.0	58.1	1860-1869 bp	1.0	80.0
ccsA_p5	GGCGGCATGTCATCTTCTAA	GTACCCGGATTTCGCTCTTT	50.0	50.0	58.0	58.0	1383-1392 bp	1.0	80.0