Hotspot

rpl32-trnL-UAG

SSC · 114669-115627 · 959 bp

Back to Trachelospermum

Pi 0.00943

MarkerSeek score 59.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 959
variable_sites: 9
pi: 0.00943
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGAAGGGATATTGGACAGCG	TTAGAAGATGACATGCCGCC	55.0	50.0	58.1	58.0	1150-1156 bp	1.0	79.7
rpl32-trnL-UAG_p2	GGAAGGGATATTGGACAGCG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.1	57.9	1096-1102 bp	1.0	79.1
rpl32-trnL-UAG_p3	GGAAGGGATATTGGACAGCG	AGCAGCGTGTCTACCAATTT	55.0	45.0	58.1	57.8	1089-1095 bp	1.0	78.6
rpl32-trnL-UAG_p4	GGAAGGGATATTGGACAGCG	CCTAAGAGCAGCGTGTCTAC	55.0	55.0	58.1	57.8	1095-1101 bp	1.0	78.5
rpl32-trnL-UAG_p5	GGAAGGGATATTGGACAGCG	CTAAGAGCAGCGTGTCTACC	55.0	55.0	58.1	57.8	1094-1100 bp	1.0	78.5