Hotspot

ndhF-rpl32

SSC · 113730-114506 · 777 bp

Back to Trachelospermum

Pi 0.0103

MarkerSeek score 55.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 777
variable_sites: 8
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ATAGGAACGGGAAGTGGGAT	CGCTGTCCAATATCCCTTCC	50.0	55.0	57.8	58.1	919-920 bp	1.0	78.3
ndhF-rpl32_p2	GAACGGGAAGTGGGATGAAA	CGCTGTCCAATATCCCTTCC	50.0	55.0	57.8	58.1	915-916 bp	1.0	78.3
ndhF-rpl32_p3	AACGGGAAGTGGGATGAAAG	CGCTGTCCAATATCCCTTCC	50.0	55.0	57.8	58.1	914-915 bp	1.0	78.3
ndhF-rpl32_p4	CCACCCGTGTTGATATGTCT	CGCTGTCCAATATCCCTTCC	50.0	55.0	57.3	58.1	887-888 bp	1.0	74.4
ndhF-rpl32_p5	ATAGGAACGGGAAGTGGGAT	ACGCTGTCCAATATCCCTTC	50.0	50.0	57.8	57.4	920-921 bp	1.0	74.3