Hotspot

rpl20-rps12

LSC · 70505-71299 · 795 bp

Back to Trachelospermum

Pi 0.00377

MarkerSeek score 60.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 795
variable_sites: 3
pi: 0.00377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTAAGTCTTGAATGGGCCCC	CTCAACGTCGAGGAACATGT	55.0	50.0	58.2	57.9	922 bp	1.0	77.8
rpl20-rps12_p2	TAAGTCTTGAATGGGCCCCT	CTCAACGTCGAGGAACATGT	50.0	50.0	58.3	57.9	921 bp	1.0	77.2
rpl20-rps12_p3	GTAAGTCTTGAATGGGCCCC	GAGGAACATGTACTCGGGTG	55.0	55.0	58.2	57.7	913 bp	1.0	76.0
rpl20-rps12_p4	GTAAGTCTTGAATGGGCCCC	AGGAACATGTACTCGGGTGT	55.0	50.0	58.2	58.4	912 bp	1.0	75.5
rpl20-rps12_p5	TAAGTCTTGAATGGGCCCCT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.3	57.7	912 bp	1.0	75.4