Hotspot

trnY-GUA-trnE-UUC

LSC · 31689-31738 · 50 bp

Back to Trachelospermum

Pi 0.04
MarkerSeek score 66.00
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
50
variable_sites
2
pi
0.04
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CCAGCGTAGACATATTGCCA AGTGGTCCAGGACATCTCTC 50.0 55.0 57.8 58.1 174 bp 1.0 77.9
trnY-GUA-trnE-UUC_p2 CCAGCGTAGACATATTGCCA GTGGTCCAGGACATCTCTCT 50.0 55.0 57.8 58.1 173 bp 1.0 77.9
trnY-GUA-trnE-UUC_p3 ACCAGCGTAGACATATTGCC AGTGGTCCAGGACATCTCTC 50.0 55.0 57.8 58.1 175 bp 1.0 77.9
trnY-GUA-trnE-UUC_p4 ACCAGCGTAGACATATTGCC GTGGTCCAGGACATCTCTCT 50.0 55.0 57.8 58.1 174 bp 1.0 77.9
trnY-GUA-trnE-UUC_p5 CCAGCGTAGACATATTGCCA TCTCTTTCAAGGAGGCAACG 50.0 50.0 57.8 57.8 158 bp 1.0 77.7