Hotspot

atpA-atpF

LSC · 11671-11725 · 55 bp

Back to Trachelospermum

Pi 0.0182

MarkerSeek score 63.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 55
variable_sites: 1
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	TTACAGGGAGCCCTAGGAAC	50.0	55.0	58.0	58.1	282 bp	1.0	79.0
atpA-atpF_p2	ATGCCATCGCCTACTTGAAG	GGAGCCCTAGGAACTCTGAA	50.0	55.0	58.0	58.1	276 bp	1.0	78.9
atpA-atpF_p3	ATGCCATCGCCTACTTGAAG	CTTACAGGGAGCCCTAGGAA	50.0	55.0	58.0	57.8	283 bp	1.0	78.5
atpA-atpF_p4	TTTCGTCAGCTCGAATGGTT	TTACAGGGAGCCCTAGGAAC	45.0	55.0	57.8	58.1	191 bp	1.0	78.1
atpA-atpF_p5	TTTCGTCAGCTCGAATGGTT	GGAGCCCTAGGAACTCTGAA	45.0	55.0	57.8	58.1	185 bp	1.0	78.1