Hotspot

rpl2

IRa · 157901-159390 · 1490 bp

Back to Toxicodendron

Pi 0.00107

MarkerSeek score 50.30

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1490
variable_sites: 4
pi: 0.00107
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGCTTCAACCGGGTTATTCT	AAAACGAAGAAAGAAATTCGATTTTCT	50.0	25.9	58.3	57.5	1588 bp	1.0	40.2
rpl2_p2	ATCATTACGCTTCAACCGGG	AAAACGAAGAAAGAAATTCGATTTTCT	50.0	25.9	58.3	57.5	1595 bp	1.0	40.2
rpl2_p3	CGCTTCAACCGGGTTATTCT	AAAACGAAGAAAGAAATTCGATTTTC	50.0	26.9	58.3	56.3	1588 bp	1.0	40.2
rpl2_p4	ATCATTACGCTTCAACCGGG	AAAACGAAGAAAGAAATTCGATTTTC	50.0	26.9	58.3	56.3	1595 bp	1.0	40.2
rpl2_p5	ACGCTTCAACCGGGTTATTC	AAAACGAAGAAAGAAATTCGATTTTCT	50.0	25.9	58.6	57.5	1589 bp	1.0	40.2