Hotspot

ndhD-psaC

SSC · 121207-121730 · 524 bp

Back to Toxicodendron

Pi 0.0112

MarkerSeek score 66.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 524
variable_sites: 14
pi: 0.0112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TTTGACCGCTGTACATTGCT	AAACAACTCGAAGCATGGGT	45.0	45.0	58.1	58.0	573-581 bp	1.0	82.0
ndhD-psaC_p2	TTTGACCGCTGTACATTGCT	GGCATGAAACAACTCGAAGC	45.0	50.0	58.1	58.0	579-587 bp	1.0	81.9
ndhD-psaC_p3	CCCCCACACGGATAGAAGTA	AAACAACTCGAAGCATGGGT	55.0	45.0	58.2	58.0	665-673 bp	1.0	80.8
ndhD-psaC_p4	CCCCCACACGGATAGAAGTA	GGCATGAAACAACTCGAAGC	55.0	50.0	58.2	58.0	671-679 bp	1.0	80.6
ndhD-psaC_p5	TTTGACCGCTGTACATTGCT	AAGAGATGTGAATCCGCCTG	45.0	50.0	58.1	57.7	634-642 bp	1.0	79.3