Hotspot

ccsA-ndhD

SSC · 119722-120045 · 324 bp

Back to Toxicodendron

Pi 0.0262

MarkerSeek score 62.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 324
variable_sites: 18
pi: 0.0262
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGATATGCTACTTCGGGGT	TTATCGATGGCTTCCCTTGC	50.0	50.0	57.9	58.0	758-778 bp	1.0	83.1
ccsA-ndhD_p2	AGCGCTATTTTTACCCAGGG	TTATCGATGGCTTCCCTTGC	50.0	50.0	57.9	58.0	1349-1369 bp	1.0	82.0
ccsA-ndhD_p3	AGCGCTATTTTTACCCAGGG	TATCTTGACGAAATGGGCGG	50.0	50.0	57.9	58.1	1412-1432 bp	1.0	81.7
ccsA-ndhD_p4	AGCGCTATTTTTACCCAGGG	ATGGGTTTATTAGCGCTGCA	50.0	45.0	57.9	57.9	1477-1497 bp	1.0	81.6
ccsA-ndhD_p5	AGCGCTATTTTTACCCAGGG	GAACTATTACCGCACGCTCA	50.0	50.0	57.9	58.1	1691-1711 bp	1.0	81.1