Hotspot

rpl32-trnL-UAG

SSC · 117609-118561 · 953 bp

Back to Toxicodendron

Pi 0.0134

MarkerSeek score 62.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 953
variable_sites: 28
pi: 0.0134
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TAGCGAAATCCCTTTCCACG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1056-1346 bp	1.0	81.7
rpl32-trnL-UAG_p2	TAGCGAAATCCCTTTCCACG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1057-1347 bp	1.0	81.7
rpl32-trnL-UAG_p3	TAGCGAAATCCCTTTCCACG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1066-1356 bp	1.0	80.8
rpl32-trnL-UAG_p4	TAGCGAAATCCCTTTCCACG	TTATAAGATGCTCTGCCGCC	50.0	50.0	58.0	57.8	1120-1410 bp	1.0	80.3
rpl32-trnL-UAG_p5	TAGCGAAATCCCTTTCCACG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	1064-1354 bp	1.0	80.2