Hotspot

rps19-rpl2

IRb · 87574-87743 · 170 bp

Back to Toxicodendron

Pi 0.0271

MarkerSeek score 61.80

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 170
variable_sites: 11
pi: 0.0271
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	CTCATAAGAACGCCCACGAA	CCGGATCGAAATGTTGGCTA	50.0	50.0	58.0	58.1	703-721 bp	1.0	82.6
rps19-rpl2_p2	TAAGAACGCCCACGAATCTG	CCGGATCGAAATGTTGGCTA	50.0	50.0	58.0	58.1	699-717 bp	1.0	82.6
rps19-rpl2_p3	TAAGAACGCCCACGAATCTG	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	715-733 bp	1.0	82.2
rps19-rpl2_p4	CTCATAAGAACGCCCACGAA	GGAAGGGCTCCAATTGGTAG	50.0	55.0	58.0	57.9	617-635 bp	0.8	72.9
rps19-rpl2_p5	TAAGAACGCCCACGAATCTG	GGAAGGGCTCCAATTGGTAG	50.0	55.0	58.0	57.9	613-631 bp	0.8	72.9