Hotspot

petG-trnW-CCA

LSC · 69443-69599 · 157 bp

Back to Toxicodendron

Pi 0.0293

MarkerSeek score 64.30

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 157
variable_sites: 9
pi: 0.0293
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	238-241 bp	1.0	82.1
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	260-263 bp	1.0	82.0
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	224-227 bp	1.0	82.0
petG-trnW-CCA_p4	ACAGACGTGGTGATCAGTTG	CCGTTGTGATAAGAAAGCGC	50.0	50.0	57.8	57.8	275-278 bp	1.0	80.7
petG-trnW-CCA_p5	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.8	57.7	258-261 bp	1.0	79.4