Hotspot

petA-psbJ

LSC · 66099-66880 · 782 bp

Back to Toxicodendron

Pi 0.00833

MarkerSeek score 65.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 782
variable_sites: 14
pi: 0.00833
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACCCATTACGTGTACAAGGC	TATTCCGGATTGGGTTCGTC	50.0	50.0	57.9	57.4	928-956 bp	1.0	75.8
petA-psbJ_p2	GCATCTGTTGTTTTGGCACA	TATTCCGGATTGGGTTCGTC	45.0	50.0	57.8	57.4	893-921 bp	1.0	74.9
petA-psbJ_p3	TTGGCATCTGTTGTTTTGGC	TATTCCGGATTGGGTTCGTC	45.0	50.0	57.8	57.4	896-924 bp	1.0	74.8
petA-psbJ_p4	ACCCATTACGTGTACAAGGC	GGATTGGGTTCGTCCTTGTA	50.0	50.0	57.9	57.2	922-950 bp	1.0	74.5
petA-psbJ_p5	GCATCTGTTGTTTTGGCACA	GGATTGGGTTCGTCCTTGTA	45.0	50.0	57.8	57.2	887-915 bp	1.0	73.6