Hotspot

trnK-UUU-rps16

LSC · 4610-5607 · 998 bp

Back to Toxicodendron

Pi 0.0176
MarkerSeek score 72.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
998
variable_sites
40
pi
0.0176
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCGATGGTATGGACGAATC TATTTCAAAGAAGGCGGGGG 55.0 50.0 57.9 57.9 1155-1250 bp 1.0 83.7
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TATTTCAAAGAAGGCGGGGG 55.0 50.0 57.9 57.9 1154-1249 bp 1.0 83.7
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TATTTCAAAGAAGGCGGGGG 50.0 50.0 58.2 57.9 1093-1188 bp 1.0 83.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TATTTCAAAGAAGGCGGGGG 50.0 50.0 58.2 57.9 1092-1187 bp 1.0 83.3
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA TATTTCAAAGAAGGCGGGGG 50.0 50.0 58.3 57.9 1102-1197 bp 1.0 82.3