Hotspot

ycf1

SSC · 125613-131114 · 5502 bp

Back to Tournefortia

Pi 0.0329

MarkerSeek score 58.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5502
variable_sites: 180
pi: 0.0329
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTATCCGCGCTTTCTCCTTT	ATCCGCCAAGTTTGTCAACT	50.0	45.0	58.1	58.0	975-978 bp	1.0	83.4
ycf1_p2	TATCCGCGCTTTCTCCTTTC	ATCCGCCAAGTTTGTCAACT	50.0	45.0	58.1	58.0	974-977 bp	1.0	83.4
ycf1_p3	TTTCTATCCGCGCTTTCTCC	ATCCGCCAAGTTTGTCAACT	50.0	45.0	58.1	58.0	978-981 bp	1.0	83.3
ycf1_p4	TCTGCTATTTCTATCCGCGC	ATCCGCCAAGTTTGTCAACT	50.0	45.0	57.9	58.0	985 bp	0.5	59.3
ycf1_p5	CTGCTATTTCTATCCGCGCT	ATCCGCCAAGTTTGTCAACT	50.0	45.0	57.9	58.0	984 bp	0.5	59.3