Hotspot

ndhF

SSC · 112036-114282 · 2247 bp

Back to Tournefortia

Pi 0.029

MarkerSeek score 59.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 65
pi: 0.029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	CACCGTGCCATAGAAATCGA	50.0	50.0	58.0	58.1	2969-2973 bp	1.0	81.6
ndhF_p2	AAGAGGGATCCACCGAAGAA	CACCGTGCCATAGAAATCGA	50.0	50.0	58.0	58.1	2961-2965 bp	1.0	81.6
ndhF_p3	AGGGATCCACCGAAGAAGAT	CACCGTGCCATAGAAATCGA	50.0	50.0	57.8	58.1	2958-2962 bp	1.0	80.5
ndhF_p4	ACAGGAACAAGAGGGATCCA	GAATCCACCGTGCCATAGAA	50.0	50.0	58.0	57.7	2974-2978 bp	1.0	79.5
ndhF_p5	ACAGGAACAAGAGGGATCCA	TCCACCGTGCCATAGAAATC	50.0	50.0	58.0	57.7	2971-2975 bp	1.0	79.5