Hotspot

ccsA-ndhD

SSC · 117144-117393 · 250 bp

Back to Tournefortia

Pi 0.0736

MarkerSeek score 75.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 250
variable_sites: 17
pi: 0.0736
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCTGCAATTGTGGCGTCTAT	AGGTCGAAGTTATTCTATCTAATTCT	45.0	30.8	57.6	55.6	391-402 bp	1.0	47.3
ccsA-ndhD_p2	TTCTGCAATTGTGGCGTCTA	AGGTCGAAGTTATTCTATCTAATTCT	45.0	30.8	57.8	55.6	392-403 bp	1.0	47.2
ccsA-ndhD_p3	TTCTGCAATTGTGGCGTCTA	AAGGTCGAAGTTATTCTATCTAA	45.0	30.4	57.8	52.5	393-404 bp	1.0	47.2
ccsA-ndhD_p4	AATTCTGCAATTGTGGCGTC	AGGTCGAAGTTATTCTATCTAATTCT	45.0	30.8	57.6	55.6	394-405 bp	1.0	47.2
ccsA-ndhD_p5	ATTCTGCAATTGTGGCGTCT	AGGTCGAAGTTATTCTATCTAATTCT	45.0	30.8	58.5	55.6	393-404 bp	1.0	47.2