Hotspot

trnL-UAG-ccsA

SSC · 116065-116174 · 110 bp

Back to Tournefortia

Pi 0.0784

MarkerSeek score 80.70

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 110
variable_sites: 8
pi: 0.0784
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	ACAATTGAAACGATCGAAAAAGA	50.0	30.4	57.9	55.7	225-233 bp	1.0	45.6
trnL-UAG-ccsA_p2	GTAGACACGCTGCTCTTAGG	ACAATTGAAACGATCGAAAAAGA	55.0	30.4	57.8	55.7	226-234 bp	1.0	45.6
trnL-UAG-ccsA_p3	AAATTGGTAGACACGCTGCT	ACAATTGAAACGATCGAAAAAGA	45.0	30.4	57.8	55.7	232-240 bp	1.0	45.4
trnL-UAG-ccsA_p4	ACAAGCCGCTATGGTGAAAT	ACAATTGAAACGATCGAAAAAGA	45.0	30.4	57.9	55.7	248-256 bp	1.0	45.1
trnL-UAG-ccsA_p5	GAGTTGAAGACAAGCCGCTA	ACAATTGAAACGATCGAAAAAGA	50.0	30.4	57.9	55.7	257-265 bp	1.0	44.9