Hotspot

trnS-GGA-rps4

LSC · 46520-46801 · 282 bp

Back to Tournefortia

Pi 0.0567

MarkerSeek score 78.30

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 282
variable_sites: 16
pi: 0.0567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	TTTGACCCTTCACCCATTCC	50.0	50.0	58.1	57.7	440 bp	1.0	79.7
trnS-GGA-rps4_p2	AGGCTTTTGTTTACCGAGGG	GACCCTTCACCCATTCCAAT	50.0	50.0	58.1	57.5	437 bp	1.0	77.9
trnS-GGA-rps4_p3	GGCTTTTGTTTACCGAGGGT	TTTGACCCTTCACCCATTCC	50.0	50.0	58.4	57.7	439 bp	1.0	77.4
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	TTTGACCCTTCACCCATTCC	55.0	50.0	58.4	57.7	492 bp	1.0	77.2
trnS-GGA-rps4_p5	TTCACTCGGTTTTTGGGTCA	TTTGACCCTTCACCCATTCC	45.0	50.0	57.6	57.7	520 bp	1.0	76.7