Hotspot

psaA-ycf3

LSC · 43053-43884 · 832 bp

Back to Tournefortia

Pi 0.0408

MarkerSeek score 76.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 31
pi: 0.0408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTGGCCCATTCTTCGAAAGA	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	878-947 bp	1.0	84.8
psaA-ycf3_p2	TCTGGCCCATTCTTCGAAAG	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	879-948 bp	1.0	84.8
psaA-ycf3_p3	GTCTGGCCCATTCTTCGAAA	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.2	57.9	880-949 bp	1.0	84.3
psaA-ycf3_p4	TTGAGAAATGACCCGGTCTG	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.5	57.9	895-964 bp	1.0	81.9
psaA-ycf3_p5	TTTTGACTTCTGGTTCCGGC	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.7	57.9	829-898 bp	1.0	80.4