Hotspot

trnS-GCU-trnG-UCC

LSC · 8521-9353 · 833 bp

Back to Tournefortia

Pi 0.0413

MarkerSeek score 75.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 833
variable_sites: 31
pi: 0.0413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	848-907 bp	1.0	58.2
trnS-GCU-trnG-UCC_p2	CTTTAGTCCACTCAGCCACC	ACGAATCACACTTTTACCACT	55.0	38.1	57.9	55.5	833-892 bp	1.0	57.8
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	897-956 bp	1.0	57.2
trnS-GCU-trnG-UCC_p4	TTTAGTCCACTCAGCCACCT	ACGAATCACACTTTTACCACT	50.0	38.1	58.3	55.5	832-891 bp	1.0	56.4
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	849-908 bp	1.0	56.3