Hotspot

psbI-trnS-GCU

LSC · 8311-8432 · 122 bp

Back to Tournefortia

Pi 0.0603

MarkerSeek score 80.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 7
pi: 0.0603
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATGATCCAGGGCGTAATCCT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.3	58.0	220-225 bp	1.0	82.2
psbI-trnS-GCU_p2	ATGATCCAGGGCGTAATCCT	TTTCAATTGGGAGAGGTGGC	50.0	50.0	58.3	58.1	249-254 bp	1.0	81.7
psbI-trnS-GCU_p3	ATGATCCAGGGCGTAATCCT	GGTGGCTGAGTGGACTAAAG	50.0	55.0	58.3	57.9	235-240 bp	1.0	81.5
psbI-trnS-GCU_p4	ATGATCCAGGGCGTAATCCT	AGGTGGCTGAGTGGACTAAA	50.0	50.0	58.3	58.3	236-241 bp	1.0	80.1
psbI-trnS-GCU_p5	AATGATCCAGGGCGTAATCC	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.4	58.0	221-226 bp	1.0	79.8