Hotspot

rps16-trnQ-UUG

LSC · 6141-7236 · 1096 bp

Back to Tournefortia

Pi 0.0542

MarkerSeek score 82.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 53
pi: 0.0542
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GCTTTCTGCCACATCGTTTC	GCTATTCGGAGGTTCGAGTC	50.0	55.0	58.0	57.9	1075-1165 bp	1.0	86.6
rps16-trnQ-UUG_p2	GCTTTCTGCCACATCGTTTC	TATTCGGAGGTTCGAGTCCT	50.0	50.0	58.0	57.6	1073-1163 bp	1.0	84.1
rps16-trnQ-UUG_p3	GCTTTCTGCCACATCGTTTC	ATTCGGAGGTTCGAGTCCTT	50.0	50.0	58.0	58.4	1072-1162 bp	1.0	84.0
rps16-trnQ-UUG_p4	CGGATCGTGTCCTTCAAGTC	GCTATTCGGAGGTTCGAGTC	55.0	55.0	58.4	57.9	1102-1192 bp	1.0	83.8
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GCTATTCGGAGGTTCGAGTC	55.0	55.0	58.4	57.9	1101-1191 bp	1.0	83.8