Hotspot

trnH-GUG-psbA

LSC · 80-358 · 279 bp

Back to Tournefortia

Pi 0.141

MarkerSeek score 78.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 279
variable_sites: 36
pi: 0.141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCGAAGCTCCATCTACAAATGG	55.0	45.5	58.1	58.2	321-326 bp	1.0	73.3
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	335-340 bp	1.0	73.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	334-339 bp	1.0	73.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	336-341 bp	1.0	73.3
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CGAAGCTCCATCTACAAATGG	55.0	47.6	58.1	56.8	320-325 bp	1.0	73.1