Hotspot

rrn16-trnI-GAU

Genome · 103125-104216 · 1092 bp

Back to Torreya

Pi 0.013

MarkerSeek score 65.60

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: Genome
length_bp: 1092
variable_sites: 28
pi: 0.013
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	1164-1192 bp	1.0	81.8
rrn16-trnI-GAU_p2	AGTGACTGGAGTGAAGTCGT	GGCACAACGACGCAATTATC	50.0	50.0	58.3	58.2	1186-1214 bp	1.0	81.6
rrn16-trnI-GAU_p3	AAGTCGTAACAAGGTAGCCG	GGCACAACGACGCAATTATC	50.0	50.0	57.6	58.2	1173-1201 bp	1.0	81.3
rrn16-trnI-GAU_p4	AACAAGGTAGCCGTACTGGA	GGCACAACGACGCAATTATC	50.0	50.0	58.4	58.2	1166-1194 bp	1.0	81.3
rrn16-trnI-GAU_p5	ACAAGGTAGCCGTACTGGAA	GGCACAACGACGCAATTATC	50.0	50.0	58.4	58.2	1165-1193 bp	1.0	81.3