Hotspot

trnV-GAC-rrn16

Genome · 100776-101613 · 838 bp

Back to Torreya

Pi 0.0574
MarkerSeek score 82.80
Primer available yes
Label trnV-GAC-rrn16

Feature Metadata

label_name
trnV-GAC-rrn16
feature_type
igs
region
Genome
length_bp
838
variable_sites
94
pi
0.0574
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rrn16_p1 TCGGTTCAAATCTGATTATCCCT CGACTTGCATGTGTTAAGCA 39.1 45.0 57.4 57.0 545-926 bp 1.0 55.2
trnV-GAC-rrn16_p2 TCGGTTCAAATCTGATTATCCCT CATGTGTTAAGCATGCCGC 39.1 52.6 57.4 58.1 538-919 bp 1.0 54.5
trnV-GAC-rrn16_p3 TCGGTTCAAATCTGATTATCCCT GAGCCAGGATCAAACTCTCA 39.1 50.0 57.4 56.9 506-887 bp 1.0 54.4
trnV-GAC-rrn16_p4 TCGGTTCAAATCTGATTATCCCT TGAGCCAGGATCAAACTCTC 39.1 50.0 57.4 56.9 507-888 bp 1.0 54.4
trnV-GAC-rrn16_p5 TCGGTTCAAATCTGATTATCCCT CTGAGCCAGGATCAAACTCT 39.1 50.0 57.4 56.9 508-889 bp 1.0 54.3