Hotspot

trnI-CAU-ycf2

Genome · 86963-87705 · 743 bp

Back to Torreya

Pi 0.0164

MarkerSeek score 59.00

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: Genome
length_bp: 743
variable_sites: 23
pi: 0.0164
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	TACCATTCAGCCATGGATGC	ACCAAAATGTTTTTCGGTGT	50.0	35.0	58.0	54.5	788-796 bp	1.0	55.1
trnI-CAU-ycf2_p2	AGGAATTGAACCCGCAACTT	ACCAAAATGTTTTTCGGTGT	45.0	35.0	58.0	54.5	831-839 bp	1.0	55.0
trnI-CAU-ycf2_p3	TACCATTCAGCCATGGATGC	ACCAAAATGTTTTTCGGTGTT	50.0	33.3	58.0	55.3	788-796 bp	1.0	53.8
trnI-CAU-ycf2_p4	AGGAATTGAACCCGCAACTT	AACCAAAATGTTTTTCGGTGT	45.0	33.3	58.0	55.3	832-840 bp	1.0	53.7
trnI-CAU-ycf2_p5	AGGAATTGAACCCGCAACTT	ACCAAAATGTTTTTCGGTGTT	45.0	33.3	58.0	55.3	831-839 bp	1.0	53.7