Hotspot

ndhC-trnV-UAC

Genome · 50208-51551 · 1344 bp

Back to Torreya

Pi 0.00856

MarkerSeek score 57.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: Genome
length_bp: 1344
variable_sites: 22
pi: 0.00856
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCCAAAGCTATGGAAAT	TGGGTTCTTGGAACAGTTCG	50.0	50.0	58.2	58.0	1527-1535 bp	1.0	79.5
ndhC-trnV-UAC_p2	GGGGCCAAAGCTATGGAAAT	TGCATGTTGGGTTCTTGGAA	50.0	45.0	58.2	57.9	1534-1542 bp	1.0	79.2
ndhC-trnV-UAC_p3	TTATCGGGGCCAAAGCTATG	TGGGTTCTTGGAACAGTTCG	50.0	50.0	57.7	58.0	1532-1540 bp	1.0	79.0
ndhC-trnV-UAC_p4	TTATCGGGGCCAAAGCTATG	TGCATGTTGGGTTCTTGGAA	50.0	45.0	57.7	57.9	1539-1547 bp	1.0	78.7
ndhC-trnV-UAC_p5	GGGGCCAAAGCTATGGAAAT	GGGTTCTTGGAACAGTTCGA	50.0	50.0	58.2	57.8	1526-1534 bp	1.0	77.8