Hotspot

trnD-GUC-psbM

Genome · 24027-25314 · 1288 bp

Back to Torreya

Pi 0.0059
MarkerSeek score 57.10
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
Genome
length_bp
1288
variable_sites
16
pi
0.0059
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTAGAGTACCGCCCTGTCAA CAATTGCTTTGACTCACCGT 50.0 45.0 58.1 56.9 1427-1455 bp 1.0 72.9
trnD-GUC-psbM_p2 ATTGGTTAGAGTACCGCCCT CAATTGCTTTGACTCACCGT 50.0 45.0 58.1 56.9 1432-1460 bp 1.0 72.5
trnD-GUC-psbM_p3 TTAGAGTACCGCCCTGTCAA TCAATTGCTTTGACTCACCG 50.0 45.0 58.1 56.7 1428-1456 bp 1.0 70.6
trnD-GUC-psbM_p4 TTAGAGTACCGCCCTGTCAA CAATTGCTTTGACTCACCGTT 50.0 42.9 58.1 57.6 1427-1455 bp 1.0 70.6
trnD-GUC-psbM_p5 ATTGGTTAGAGTACCGCCCT TCAATTGCTTTGACTCACCG 50.0 45.0 58.1 56.7 1433-1461 bp 1.0 70.2