Hotspot

rpoA

LSC · 81873-82868 · 996 bp

Back to Torminalis

Pi 0

MarkerSeek score 58.80

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 996
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TCGGAATATATGGATTGTGCT	CCACATAATGGCTGTAGGC	38.1	52.6	54.6	55.7	1209-1210 bp	1.0	40.1
rpoA_p2	TTCGGAATATATGGATTGTGCT	CCACATAATGGCTGTAGGC	36.4	52.6	55.4	55.7	1210-1211 bp	1.0	40.1
rpoA_p3	TGGATTGTGCTAAAGATTCAAGA	CCACATAATGGCTGTAGGC	34.8	52.6	56.2	55.7	1199-1200 bp	1.0	40.1
rpoA_p4	TGGACCTATTCGGAATATATGGA	CCACATAATGGCTGTAGGC	39.1	52.6	56.1	55.7	1218-1219 bp	1.0	40.1
rpoA_p5	TCGGAATATATGGATTGTGC	CCACATAATGGCTGTAGGC	40.0	52.6	52.9	55.7	1209-1210 bp	1.0	40.1