Hotspot

psbZ-trnG-UCC

LSC · 38902-39351 · 450 bp

Back to Torminalis

Pi 0

MarkerSeek score 57.60

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	599-608 bp	1.0	77.6
psbZ-trnG-UCC_p2	GGGTTGGTCGGGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	585-594 bp	1.0	77.4
psbZ-trnG-UCC_p3	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.0	57.8	555-564 bp	1.0	71.8
psbZ-trnG-UCC_p4	AGGGTTGGTCGGGTAACAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	59.2	57.8	586-595 bp	1.0	70.6
psbZ-trnG-UCC_p5	ATTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.2	57.8	600-609 bp	1.0	70.2