Hotspot

rps16-trnQ-UUG

LSC · 6529-7327 · 799 bp

Back to Torminalis

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
799
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 883 bp 1.0 77.8
rps16-trnQ-UUG_p2 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 893 bp 1.0 76.1
rps16-trnQ-UUG_p3 GCTTTCTGCCACATCGTTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 860 bp 1.0 76.0
rps16-trnQ-UUG_p4 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 894 bp 1.0 76.0
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GTGTCGACTAGAAATGGGGC 50.0 55.0 58.2 58.3 945 bp 1.0 75.6