Hotspot

matK-trnK-UUU

LSC · 3697-4400 · 704 bp

Back to Torminalis

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCATGATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.2	58.2	835 bp	1.0	72.4
matK-trnK-UUU_p2	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.2	58.2	834 bp	1.0	72.4
matK-trnK-UUU_p3	CCATGATCATGAGCAAGTGC	TTGGTTTGGATTGCGTTGTG	50.0	45.0	57.2	57.8	929 bp	1.0	72.1
matK-trnK-UUU_p4	CCATGATCATGAGCAAGTGC	TTGGGTTGCTAACTCAACGG	50.0	50.0	57.2	58.4	850 bp	1.0	70.6
matK-trnK-UUU_p5	CCATGATCATGAGCAAGTGC	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.2	57.5	848 bp	1.0	70.1