Hotspot

petB

LSC · 75938-77324 · 1387 bp

Back to Torenia

Pi 0.00948
MarkerSeek score 50.00
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1387
variable_sites
44
pi
0.00948
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 TGAAGTAATGAGCCTCCCCA TCTGGGTCGTCTCAGACTTT 50.0 50.0 58.0 58.0 2662-2678 bp 1.0 83.6
petB_p2 TGAAGTAATGAGCCTCCCCA TTCTGGGTCGTCTCAGACTT 50.0 50.0 58.0 58.0 2663-2679 bp 1.0 83.6
petB_p3 TGGGGAGGCTCATTACTTCA TCTGGGTCGTCTCAGACTTT 50.0 50.0 58.0 58.0 2639-2655 bp 1.0 83.5
petB_p4 TGGGGAGGCTCATTACTTCA TTCTGGGTCGTCTCAGACTT 50.0 50.0 58.0 58.0 2640-2656 bp 1.0 83.5
petB_p5 TGGGGAGGCTCATTACTTCA TTTCTGGGTCGTCTCAGACT 50.0 50.0 58.0 58.0 2641-2657 bp 1.0 83.5