Hotspot

ycf1-trnN-GUU

IRa · 128628-129174 · 547 bp

Back to Torenia

Pi 0.0163

MarkerSeek score 70.50

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 547
variable_sites: 44
pi: 0.0163
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	GACGGCCGAATTGGTTATCT	AGGATTCGAACCTACGACCA	50.0	50.0	58.1	58.2	563-692 bp	0.916667	84.7
ycf1-trnN-GUU_p2	GACGGCCGAATTGGTTATCT	CCCCAAGTAGGATTCGAACC	50.0	55.0	58.1	57.7	571-700 bp	0.916667	83.3
ycf1-trnN-GUU_p3	ACGGCCGAATTGGTTATCTT	AGGATTCGAACCTACGACCA	45.0	50.0	57.6	58.2	562-691 bp	0.916667	81.8
ycf1-trnN-GUU_p4	GACGGCCGAATTGGTTATCT	GGATTCGAACCTACGACCAA	50.0	50.0	58.1	57.3	562-691 bp	0.916667	80.7
ycf1-trnN-GUU_p5	ACGGCCGAATTGGTTATCTT	CCCCAAGTAGGATTCGAACC	45.0	55.0	57.6	57.7	570-699 bp	0.916667	80.4