Hotspot

rpl32-trnL-UAG

SSC · 112621-113447 · 827 bp

Back to Torenia

Pi 0.0182

MarkerSeek score 65.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 827
variable_sites: 62
pi: 0.0182
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCGTTAAAAGCATTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	957 bp	0.083333	42.6
rpl32-trnL-UAG_p2	GGGCAGCGTTAAAAGCATTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	958 bp	0.083333	42.6
rpl32-trnL-UAG_p3	GGGCAGCGTTAAAAGCATTT	TTTAGGAGATGACATGCCGC	45.0	50.0	57.9	58.0	1022 bp	0.083333	42.3
rpl32-trnL-UAG_p4	GGGCAGCGTTAAAAGCATTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	967 bp	0.083333	41.7
rpl32-trnL-UAG_p5	GGGCAGCGTTAAAAGCATTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.9	57.8	965 bp	0.083333	41.1