Hotspot

petA-psbJ

LSC · 64110-65167 · 1058 bp

Back to Torenia

Pi 0.0163

MarkerSeek score 59.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1058
variable_sites: 60
pi: 0.0163
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1099-1118 bp	1.0	82.4
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1101-1120 bp	1.0	82.4
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	1098-1117 bp	1.0	81.5
petA-psbJ_p4	GAGAAGGTTCAATTGGCCGA	TTCCGGATTAGGTTCATCCCT	50.0	47.6	58.2	58.2	1100-1119 bp	1.0	76.4
petA-psbJ_p5	TCGAGAAGGTTCAATTGGCC	TTCCGGATTAGGTTCATCCCT	50.0	47.6	58.2	58.2	1102-1121 bp	1.0	76.4