Hotspot

trnM-CAU-atpE

LSC · 53639-53866 · 228 bp

Back to Torenia

Pi 0.0349
MarkerSeek score 64.40
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
228
variable_sites
25
pi
0.0349
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 GGCAGGAGTCATTGGTTCAA AGGCTGTCAATGCGATTTCA 50.0 45.0 58.1 58.2 286-330 bp 1.0 85.0
trnM-CAU-atpE_p2 GGCAGGAGTCATTGGTTCAA CTAGGACAAGAGTGGAGGCT 50.0 55.0 58.1 58.1 301-345 bp 1.0 84.9
trnM-CAU-atpE_p3 GGCAGGAGTCATTGGTTCAA AGCTAGGACAAGAGTGGAGG 50.0 55.0 58.1 58.1 303-347 bp 1.0 84.8
trnM-CAU-atpE_p4 GGCAGGAGTCATTGGTTCAA GGCAAATCTAGCTCTCCGAC 50.0 55.0 58.1 58.1 324-368 bp 0.916667 80.4
trnM-CAU-atpE_p5 GGCAGGAGTCATTGGTTCAA GAGGCAAATCTAGCTCTCCG 50.0 55.0 58.1 57.9 326-370 bp 0.916667 80.3