Hotspot

trnV-UAC-trnM-CAU

LSC · 53378-53565 · 188 bp

Back to Torenia

Pi 0.00766
MarkerSeek score 47.10
Primer available yes
Label trnV-UAC-trnM-CAU

Feature Metadata

label_name
trnV-UAC-trnM-CAU
feature_type
igs
region
LSC
length_bp
188
variable_sites
7
pi
0.00766
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-trnM-CAU_p1 ACCAACTGAGCTATAGCCCT TTGAACCAATGACTCCTGCC 50.0 50.0 57.8 58.1 259-328 bp 1.0 83.7
trnV-UAC-trnM-CAU_p2 ACCAACTGAGCTATAGCCCT CTCCTGCCGTATGAAAGCAA 50.0 50.0 57.8 58.3 247-316 bp 1.0 82.4
trnV-UAC-trnM-CAU_p3 ACCAACTGAGCTATAGCCCT CCAATGACTCCTGCCGTATG 50.0 55.0 57.8 58.4 254-323 bp 1.0 81.2
trnV-UAC-trnM-CAU_p4 ACCAACTGAGCTATAGCCCT TCCTGCCGTATGAAAGCAAT 50.0 45.0 57.8 57.6 246-315 bp 1.0 81.2
trnV-UAC-trnM-CAU_p5 CGTGTAAACGAGGTGCTCTA TTGAACCAATGACTCCTGCC 50.0 50.0 57.4 58.1 278-347 bp 1.0 80.1