Hotspot

ndhC-trnV-UAC

LSC · 51587-52722 · 1136 bp

Back to Torenia

Pi 0.0144

MarkerSeek score 59.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1136
variable_sites: 70
pi: 0.0144
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AATCGGGGCTAAAATACCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1253-1306 bp	1.0	82.8
ndhC-trnV-UAC_p2	GGGGCTAAAATACCGGCAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	1249-1302 bp	1.0	82.5
ndhC-trnV-UAC_p3	CCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.6	57.8	1262-1315 bp	1.0	80.3
ndhC-trnV-UAC_p4	AATCGGGGCTAAAATACCGG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	1252-1305 bp	1.0	80.1
ndhC-trnV-UAC_p5	GGGGCTAAAATACCGGCAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.3	57.5	1248-1301 bp	1.0	79.8